2AUA
Structure of BC2332: A Protein of Unknown Function from Bacillus cereus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.222, 76.640, 107.503 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.350 |
| R-factor | 0.20632 |
| Rwork | 0.204 |
| R-free | 0.25388 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.258 |
| Data scaling software | SCALEPACK |
| Phasing software | ACRS ((HYSS) |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.073 | 0.440 |
| Number of reflections | 24016 | |
| <I/σ(I)> | 14.3 | 3.3 |
| Completeness [%] | 99.2 | 99.9 |
| Redundancy | 4.2 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 0.1M Sodium citrate, 1M Ammonium dihydrogen Phosphate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
