2ATJ
RECOMBINANT HORSERADISH PEROXIDASE COMPLEX WITH BENZHYDROXAMIC ACID
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 283 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-03 |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.920, 62.250, 77.990 |
| Unit cell angles | 90.00, 104.36, 90.00 |
Refinement procedure
| Resolution | 28.600 * - 2.000 |
| R-factor | 0.176 |
| Rwork | 0.176 |
| R-free | 0.19800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sch |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.090 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.085 | 0.209 * |
| Total number of observations | 144839 * | |
| Number of reflections | 46595 | |
| Completeness [%] | 98.5 | 92.8 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | 283 * | pH 6.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | BHA | 0.35 (mM) | |
| 2 | 1 | drop | HRPC | 6.0 (mg/ml) | |
| 3 | 1 | reservoir | 1.0 (M) | ||
| 4 | 1 | reservoir | cacodylate | 0.1 (M) |
