2AT8
0.96 A Crystal Structure Of Nitrophorin 4 From Rhodnius Prolixus Containing Fe(III) 2,4 Dimethyl Deuteroporphyrin IX Complexed With Nitric Oxide at pH 5.6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-22 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.211, 42.582, 52.935 |
| Unit cell angles | 90.00, 94.61, 90.00 |
Refinement procedure
| Resolution | 21.290 - 1.000 |
| R-factor | 0.166 |
| Rwork | 0.165 |
| R-free | 0.19500 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1x8o |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.747 |
| Data scaling software | d*TREK (7.2SSIbeta) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.290 | 0.990 |
| High resolution limit [Å] | 0.960 | 0.960 |
| Rmerge | 0.080 | 0.410 |
| Number of reflections | 81981 | |
| <I/σ(I)> | 22.6 | 2.9 |
| Completeness [%] | 86.4 | 89 |
| Redundancy | 3.16 | 1.54 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 300 | ammonium phosphate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 300.0K |
