2AT0
1.00 A Crystal Structure Of L133V Mutant of Nitrophorin 4 From Rhodnius Prolixus Complexed With Nitric Oxide at pH 5.6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-20 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 70.286, 42.699, 52.970 |
| Unit cell angles | 90.00, 94.27, 90.00 |
Refinement procedure
| Resolution | 36.470 - 1.000 |
| R-factor | 0.14 |
| Rwork | 0.139 |
| R-free | 0.15400 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1x8o |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.489 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.510 | 1.050 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.076 | 0.178 |
| Number of reflections | 83901 | |
| <I/σ(I)> | 13.9 | 5.3 |
| Completeness [%] | 99.8 | 98 |
| Redundancy | 4.1 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 300 | ammonium phosphate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 300.0K |
