2AS0
Crystal Structure of PH1915 (APC 5817): A Hypothetical RNA Methyltransferase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.97934 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 70.513, 86.161, 76.693 |
Unit cell angles | 90.00, 114.16, 90.00 |
Refinement procedure
Resolution | 28.980 - 1.800 |
R-factor | 0.207 |
Rwork | 0.207 |
R-free | 0.23900 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 142280 | |
<I/σ(I)> | 23 | 2.5 |
Redundancy | 4.7 | 4.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.2 | 298 | 1.6 M Ammonium Sulfate, 16 % Glycerol, 0.1 M Sodium Acetate, pH 3.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |