2ARQ
Human plasminogen activator inhibitor-2.[loop (66-98) deletion mutant] complexed with peptide n-acetyl-teaaagdggvmtgr-oh
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-10-09 |
| Detector | MAC Science DIP-2030 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 92.562, 104.126, 41.438 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.800 - 1.850 |
| R-factor | 0.18469 |
| Rwork | 0.182 |
| R-free | 0.24822 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jrr |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.708 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.800 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.069 | 0.417 |
| Number of reflections | 33539 | |
| <I/σ(I)> | 15.2 | 2.1 |
| Completeness [%] | 96.2 | 98.1 |
| Redundancy | 3.9 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG 8K, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
