2ARK
Structure of a flavodoxin from Aquifex aeolicus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2005-05-06 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97911, 0.97924 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 112.846, 112.846, 150.648 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.000 - 2.400 |
| R-factor | 0.17396 |
| Rwork | 0.171 |
| R-free | 0.23519 |
| Structure solution method | MAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.717 |
| Data scaling software | HKL-2000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 43827 | |
| <I/σ(I)> | 1.8 | |
| Completeness [%] | 99.6 | 98.8 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | Na-HEPES, Na Citrate, glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
