2ARJ
CD8alpha-alpha in complex with YTS 105.18 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-01-31 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.97960 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.344, 107.135, 131.522 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.000 - 2.880 |
| R-factor | 0.22326 |
| Rwork | 0.220 |
| R-free | 0.27709 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 1ACY for YTS 105.18 Fab PDB Entry: 1BQH for CD8 component |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.684 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.629 | 3.000 |
| High resolution limit [Å] | 2.880 | 2.884 |
| Rmerge | 0.154 | 0.690 |
| Number of reflections | 26797 | |
| <I/σ(I)> | 10.8 | 1775 |
| Completeness [%] | 99.1 | 95.7 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.1 | 299 | 20% PEG 3,000, 0.1M Sodium Acetate, pH 5.1, VAPOR DIFFUSION, temperature 299K |
