2APQ
Crystal Structure of an Active Site Mutant of Bovine Pancreatic Ribonuclease A (H119A-RNase A) with a 10-Glutamine expansion in the C-terminal hinge-loop.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-04-01 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 67.228, 67.228, 63.686 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.220 - 1.800 |
| R-factor | 0.18117 |
| Rwork | 0.179 |
| R-free | 0.21465 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fs3 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.289 |
| Data reduction software | CrystalClear |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 14971 | |
| <I/σ(I)> | 36.41 | 6.2 |
| Completeness [%] | 94.4 | 91.5 |
| Redundancy | 9.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 293 | 0.4M Potassium Sodium tartrate tetrahydrate, pH 7.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
