2APO
Crystal Structure of the Methanococcus jannaschii Cbf5 Nop10 Complex
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-10 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.957, 0.9798 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 118.289, 63.189, 67.896 |
| Unit cell angles | 90.00, 102.62, 90.00 |
Refinement procedure
| Resolution | 49.170 - 1.950 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| R-free | 0.23000 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 35760 | |
| Completeness [%] | 94.6 | 70.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 295 | 2-propanol, PEG 4000, sodium citrate, potassium chloride, pH 5.8, VAPOR DIFFUSION, SITTING DROP, temperature 295.0K |
