2AP1
Crystal structure of the putative regulatory protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-21 |
| Detector | SBC-3 |
| Wavelength(s) | 1.284 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 81.581, 105.744, 88.262 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.1694 |
| Rwork | 0.168 |
| R-free | 0.20508 |
| Structure solution method | SAD |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.643 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.049 | 0.232 |
| Number of reflections | 30297 | |
| <I/σ(I)> | 15.1 | 7.9 |
| Completeness [%] | 99.6 | 99.9 |
| Redundancy | 6.8 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | HEPES, tri-Sodium Citrate, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
