2AMV
THE STRUCTURE OF GLYCOGEN PHOSPHORYLASE B WITH AN ALKYL-DIHYDROPYRIDINE-DICARBOXYLIC ACID
Replaces: 1AMVExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-11-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 127.110, 127.110, 115.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.28200 |
| Structure solution method | OTHER |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.095 | 0.382 |
| Total number of observations | 242759 * | |
| Number of reflections | 39549 | |
| <I/σ(I)> | 10.5 | 3.6 |
| Completeness [%] | 94.9 | 95.9 |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.7 | 16 * | PHOSPHORYLASE B WAS COCRYSTALLISED WITH 1 MM W1807 IN A MEDIUM CONSISTING OF 27-28 MG/ML ENZYME, 1 MM SPERMINE, 3 MM DTT, 10 MM BES, 0.1 MM EDTA, AND 0.02% SODIUM AZIDE, PH 6.7 (16 DEG C)., temperature 289K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | W1807 | 1 (mM) | |
| 2 | 1 | 1 | enzyme | 27-28 (mg/ml) | |
| 3 | 1 | 1 | spermine | 1 (mM) | |
| 4 | 1 | 1 | dithiothreitol | 3 (mM) | |
| 5 | 1 | 1 | BES | 10 (mM) | |
| 6 | 1 | 1 | EDTA | 0.1 (mM) | |
| 7 | 1 | 1 | sodium azide | 0.02 (%) |
