2AMO

Loose Dimer of a Bacillus subtilis Nitric Oxide Synthase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	193
Detector technology	CCD
Collection date	2001-10-22
Detector	ADSC QUANTUM 4
Wavelength(s)	0.92
Spacegroup name	P 21 21 2
Unit cell lengths	81.314, 93.211, 118.546
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.620 - 2.600
R-factor	0.2756
Rwork	0.274
R-free	0.28969
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1m7z
RMSD bond length	0.010
RMSD bond angle	1.812
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.1.9999)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.650
High resolution limit [Å]	2.600	2.600
Number of reflections	23234
Completeness [%]	98.4	93.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	Na Cacoldylate, Potassium Acetate, Peg4K, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	Na Cacoldylate, Potassium Acetate, Peg4K, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	Na Cacoldylate, Potassium Acetate, Peg4K, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K