2AM4

Crystal Structure of N-Acetylglucosaminyltransferase I in Complex with UDP-2-deoxy-2-fluoro-glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-02-23
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9363
Spacegroup name	P 21 21 21
Unit cell lengths	40.917, 82.501, 102.394
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.800 - 1.700
Rwork	0.193
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fo9 1foa
RMSD bond length	0.008
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.200	1.810
High resolution limit [Å]	1.670	1.700
Number of reflections	37235
Completeness [%]	95.5	84.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.9	293	40% PEK 6000, 1M Tris, MnCl2, pH 7.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K