2ALE
Crystal structure of yeast RNA splicing factor Snu13p
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-01-15 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 70.330, 70.330, 53.303 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.680 - 1.800 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e7k |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.200 |
| Data reduction software | CrystalClear ((MSC/RIGAKU)) |
| Data scaling software | d*TREK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.530 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.095 | 0.326 |
| Number of reflections | 14642 | |
| <I/σ(I)> | 11.8 | 2.8 |
| Completeness [%] | 99.4 | 99 |
| Redundancy | 5.2 | 5.15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2 M Sodium Acetate, 0.1 M Sodium Cacodylate, 30% PEG 8K, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
