2AJ4
Crystal structure of Saccharomyces cerevisiae Galactokinase in complex with galactose and Mg:AMPPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 290 |
| Detector technology | CCD |
| Collection date | 2005-03-06 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.300, 85.000, 112.300 |
| Unit cell angles | 90.00, 96.70, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.400 |
| R-factor | 0.174 |
| Rwork | 0.172 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2a2c |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.330 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | EPMR |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.500 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 44350 | |
| <I/σ(I)> | 11.2 | 2.9 |
| Completeness [%] | 95.5 | 86 |
| Redundancy | 3.4 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 294 | PEG8000, LiCl, galactose, Mg:AMPPNP, MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
