2AIP
Crystal structure of native protein C activator from the venom of copperhead snake Agkistrodon contortrix contortrix
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE D03B-MX1 |
| Synchrotron site | LNLS |
| Beamline | D03B-MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-19 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.438 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 79.867, 63.295, 48.237 |
| Unit cell angles | 90.00, 99.80, 90.00 |
Refinement procedure
| Resolution | 19.030 - 1.650 |
| R-factor | 0.17086 |
| Rwork | 0.169 |
| R-free | 0.19692 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bqy |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.493 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.030 | 1.710 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.090 | |
| Number of reflections | 28664 | |
| Completeness [%] | 99.8 | 99.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | ammonium sulfate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
