2AII
wild-type Formylglycine generating enzyme reacted with iodoacetamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-01-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 62.058, 109.788, 43.461 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.480 - 1.540 |
| R-factor | 0.15163 |
| Rwork | 0.150 |
| R-free | 0.17598 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1y1e |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.306 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.2.0009) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.600 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Number of reflections | 44155 | |
| <I/σ(I)> | 41.5 | 4.9 |
| Completeness [%] | 98.5 | 85.5 |
| Redundancy | 6.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | PEG 4000, Calcium chloride, TRIS, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
