2AI2
Purine nucleoside phosphorylase from calf spleen
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 1998-08-02 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9231 |
Spacegroup name | P 21 3 |
Unit cell lengths | 93.243, 93.243, 93.243 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.070 - 1.700 |
R-factor | 0.196 |
Rwork | 0.196 |
R-free | 0.23100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a9p |
RMSD bond length | 0.007 |
RMSD bond angle | 1.500 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 28798 | |
<I/σ(I)> | 23.1 | 5.3 |
Completeness [%] | 99.8 | 100 |
Redundancy | 6.7 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 296 | PEG2K MME , pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 296K |