2AHY
Na+ complex of the NaK Channel
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-11 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 81.528, 85.482, 129.572 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.700 - 2.400 |
R-factor | 0.236 |
Rwork | 0.236 |
R-free | 0.26100 |
Structure solution method | SIRAS |
RMSD bond length | 0.006 |
RMSD bond angle | 1.140 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.700 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.045 | 0.555 |
Number of reflections | 17824 | |
<I/σ(I)> | 35 | 1.8 |
Completeness [%] | 97.6 | 84.1 |
Redundancy | 5 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | 293 | PEG 400, NaCl, CaCl2, Tris.HCl, t-butanol, n-Decyl-beta-D-maltopyranoside, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 293 K, pH 8.00 |