2AHP
GCN4 leucine zipper, mutation of Lys15 to epsilon-azido-Lys
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-07-01 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 100.162, 30.397, 21.716 |
Unit cell angles | 90.00, 95.21, 90.00 |
Refinement procedure
Resolution | 29.080 - 2.000 |
R-factor | 0.192 |
Rwork | 0.189 |
R-free | 0.26300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.204 |
Data reduction software | CrystalClear ((MSC/RIGAKU)) |
Data scaling software | d*TREK (8.0SSI) |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.080 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.036 | 0.140 |
Number of reflections | 4473 | |
<I/σ(I)> | 10.6 | 3.4 |
Completeness [%] | 98.7 | 98 |
Redundancy | 3.49 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 295 | 0.065 M Tris, 5.2% w/v PEG 8000, 35% v/v anhydrous glycerol, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |