2AHI
Structural Basis of DNA Recognition by p53 Tetramers (complex III)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-10-11 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.552, 58.190, 77.545 |
| Unit cell angles | 83.08, 87.94, 73.56 |
Refinement procedure
| Resolution | 38.490 - 1.850 |
| R-factor | 0.166 |
| Rwork | 0.162 |
| R-free | 0.22500 |
| Structure solution method | ISOMORPHOUS REPLACEMENT |
| Starting model (for MR) | 2ac0 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.666 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.880 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.045 | 0.350 |
| Number of reflections | 73053 | |
| <I/σ(I)> | 28.7 | 3.5 |
| Completeness [%] | 93.9 | 82.6 |
| Redundancy | 3.9 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 293 | Ammonium Chloride, PEG 3350, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Ammonium Chloride | ||
| 2 | 1 | 1 | PEG 3350 | ||
| 3 | 1 | 1 | H2O | ||
| 4 | 1 | 2 | Ammonium Chloride | ||
| 5 | 1 | 2 | PEG 3350 | ||
| 6 | 1 | 2 | H2O |
