2AGO

Fidelity of Dpo4: effect of metal ions, nucleotide selection and pyrophosphorolysis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	2004-12-29
Detector	RIGAKU RAXIS IV
Spacegroup name	P 21 21 2
Unit cell lengths	99.044, 101.939, 53.017
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.850
R-factor	0.22
Rwork	0.220
R-free	0.26300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jx4
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.850
Number of reflections	12652

Crystallization Conditions

crystal ID	method	pH	temperature	details
1