2AF5
2.5A X-ray Structure of Engineered OspA protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.547, 55.330, 70.361 |
| Unit cell angles | 90.00, 104.08, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.24572 |
| Rwork | 0.242 |
| R-free | 0.27556 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1osp |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.481 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.075 |
| Number of reflections | 14271 |
| <I/σ(I)> | 9.2 |
| Completeness [%] | 99.9 |
| Redundancy | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
