2AEQ

An epidemiologically significant epitope of a 1998 influenza virus neuraminidase forms a highly hydrated interface in the NA-antibody complex.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	2003-10-03
Detector	RIGAKU RAXIS IV
Spacegroup name	P 4 21 2
Unit cell lengths	159.753, 159.753, 104.113
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.820 - 3.000
R-factor	0.267
Rwork	0.267
R-free	0.31200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1nn2 1nca
RMSD bond length	0.009
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.620
High resolution limit [Å]	3.000	3.500
Number of reflections	23223
<I/σ(I)>	10.04	3.28
Completeness [%]	93.7	93.7
Redundancy	2.96	2.73

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7.5		pH 7.5