2AEO
Crystal structure of cisplatinated bovine Cu,Zn superoxide dismutase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCED ULTRA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-01 |
| Detector | OXFORD ONYX CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.207, 50.920, 146.738 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.900 - 1.800 |
| R-factor | 0.20545 |
| Rwork | 0.202 |
| R-free | 0.23601 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1sxs |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.439 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 147.440 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.170 | 0.420 |
| Number of reflections | 32303 | |
| <I/σ(I)> | 4.1 | 1.7 |
| Completeness [%] | 96.1 | 81.4 |
| Redundancy | 4.8 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 50mM Tris HCl, 18% PEG 4000, 20% 2-propanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
