2AEN
Crystal structure of the rotavirus strain DS-1 VP8* core
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-02-13 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9160 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.421, 84.123, 90.769 |
| Unit cell angles | 90.03, 90.02, 75.54 |
Refinement procedure
| Resolution | 14.880 - 1.604 |
| R-factor | 0.15961 |
| Rwork | 0.158 |
| R-free | 0.19173 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kqr |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.310 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.880 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 153466 | |
| <I/σ(I)> | 17.2 | 3.4 |
| Completeness [%] | 96.8 | 94.7 |
| Redundancy | 1.95 | 1.72 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | PEG 4000, sodium chloride, sodium citrate, ethanol, tris, EDTA, sodium azide, benzamidine, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
