2AE6
Crystal Structure of Acetyltransferase of GNAT family from Enterococcus faecalis V583
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-02-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 32 |
| Unit cell lengths | 64.618, 64.618, 176.396 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 58.820 - 2.190 |
| R-factor | 0.2152 |
| Rwork | 0.210 |
| R-free | 0.25929 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.323 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.820 | 2.280 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.081 | 0.613 |
| Number of reflections | 40808 | |
| <I/σ(I)> | 12.7 | 1.8 |
| Completeness [%] | 96.9 | 90.3 |
| Redundancy | 9.8 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | ETHANOL, HEPES, Magnesium Chloride, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
