2AE5
Glutaryl 7-Aminocephalosporanic Acid Acylase: mutational study of activation mechanism
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 4A |
| Synchrotron site | PAL/PLS |
| Beamline | 4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-23 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.95 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 73.736, 73.736, 383.349 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.240 |
| Rwork | 0.173 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.231 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.350 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.095 | 0.453 |
| Number of reflections | 57515 | |
| Completeness [%] | 90.5 | |
| Redundancy | 5.7 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | PEG 8000, Tris, magnesium chloride, cacodylate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
