2ADO

Crystal Structure Of The Brct Repeat Region From The Mediator of DNA damage checkpoint protein 1, MDC1

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2004-07-29
Detector	ADSC QUANTUM 210
Wavelength(s)	1.1159,0.9795471,1.019876
Spacegroup name	P 1
Unit cell lengths	42.051, 44.440, 61.942
Unit cell angles	72.95, 87.54, 61.80

Refinement procedure

Resolution	30.000 - 1.450
Rwork	0.195
R-free	0.21200
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE (2.08)
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.500
High resolution limit [Å]	1.450	1.450
Rmerge	0.048	0.380
Number of reflections	60634
Completeness [%]	90.8	75.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	PEG 8000, HEPES, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K
1	VAPOR DIFFUSION, HANGING DROP	8	298	PEG 8000, HEPES, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K