Experimental procedure
Detector technology | IMAGE PLATE |
Collection date | 1994-04 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 130.660, 141.830, 53.150 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.600 |
R-factor | 0.214 |
Rwork | 0.214 |
R-free | 0.27300 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 * |
Data reduction software | DENZO |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 10.000 * |
High resolution limit [Å] | 2.400 * |
Rmerge | 0.066 |
Total number of observations | 220712 * |
Number of reflections | 37305 |
Completeness [%] | 94.9 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.3 * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | M.TaqI | 0.00065 (mM) | |
2 | 1 | drop | Sinefungin | 1 (mM) | can be replaced by AdoHcy |
3 | 1 | drop | duplex oligonucleotides | 0.1 (mM) | |
4 | 1 | drop | 200 (mM) | ||
5 | 1 | drop | Tris-HCl | 20 (mM) | |
6 | 1 | reservoir | PEG6000 | 2-6 (%) | |
7 | 1 | reservoir | 100 (mM) | ||
8 | 1 | reservoir | Tris-HCl | 20 (mM) |