2AD8
crystal structure of methanol dehydrogenase from M. W3A1 (form C) in the presence of ethanol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2002-12-02 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 204.205, 62.630, 124.511 |
| Unit cell angles | 90.00, 125.14, 90.00 |
Refinement procedure
| Resolution | 42.990 - 1.600 |
| R-factor | 0.174 |
| Rwork | 0.174 |
| R-free | 0.19500 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.046 | 0.219 |
| Number of reflections | 157861 | |
| Completeness [%] | 93.3 | 68.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | macroseeding | 8.25 | 293 | Tris-HCl, PEG 8000, ethanol, pH 8.25, macroseeding, temperature 293K |
