2AC0
Structural Basis of DNA Recognition by p53 Tetramers (complex I)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-09-04 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.439, 58.146, 77.539 |
| Unit cell angles | 82.93, 87.99, 73.60 |
Refinement procedure
| Resolution | 33.520 - 1.800 |
| R-factor | 0.157 |
| Rwork | 0.153 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tsr |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.754 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.055 | 0.342 |
| Number of reflections | 79253 | |
| <I/σ(I)> | 24.5 | 4 |
| Completeness [%] | 94.2 | 90.6 |
| Redundancy | 4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.6 | 293 | Ammonium Formate, PEG 3350, pH 6.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Ammonium Formate | ||
| 2 | 1 | 1 | PEG 3350 | ||
| 3 | 1 | 1 | H2O | ||
| 4 | 1 | 2 | Ammonium Formate | ||
| 5 | 1 | 2 | PEG 3350 | ||
| 6 | 1 | 2 | H2O |
