2AAO

Regulatory apparatus of Calcium Dependent protein kinase from Arabidopsis thaliana

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2003-05-20
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9766, 0.9795, 0.95
Spacegroup name	P 21 21 21
Unit cell lengths	35.968, 88.154, 117.552
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.700 - 2.000
R-factor	0.278
Rwork	0.213
R-free	0.25400
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.308
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.030
High resolution limit [Å]	2.000	2.000
Rmerge	0.095	0.167
Number of reflections	25329
<I/σ(I)>	19.5	2.6
Completeness [%]	97.3	86.7
Redundancy	9.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.6	293	PEG400, calcium chloride, sodium acetate , pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K