2AAM

Crystal structure of a putative glycosidase (tm1410) from thermotoga maritima at 2.20 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2005-05-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979170, 0.918370
Spacegroup name	P 1 21 1
Unit cell lengths	194.930, 84.620, 195.010
Unit cell angles	90.00, 119.90, 90.00

Refinement procedure

Resolution	28.590 - 2.200
Rwork	0.216
R-free	0.23900
Structure solution method	MAD
RMSD bond length	0.016
RMSD bond angle	1.529
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	28.590	28.590	2.280
High resolution limit [Å]	2.200	4.720	2.200
Rmerge	0.093	0.543	0.543
Number of reflections	263407	53751	34138
<I/σ(I)>	8.15	17.44	1.97
Completeness [%]	90.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION,SITTING DROP,NANODROP	9.5	277	25.0% PEG-300, 10.0% Glycerol, 5.0% PEG-8000, 0.1M CHES pH 9.5 , VAPOR DIFFUSION,SITTING DROP,NANODROP, temperature 277K