2AAC

ESCHERCHIA COLI GENE REGULATORY PROTEIN ARAC COMPLEXED WITH D-FUCOSE

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-09
Detector	FUJI
Spacegroup name	P 1 21 1
Unit cell lengths	39.247, 93.682, 49.915
Unit cell angles	90.00, 95.57, 90.00

Refinement procedure

Resolution	7.000 - 1.600
R-factor	0.176
Rwork	0.176
R-free	0.22200
Structure solution method	RIGID-BODY REFINEMENT AND DIFFERENCE FOURIER MAPS
Starting model (for MR)	2arc
RMSD bond length	0.010
RMSD bond angle	24.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.670
High resolution limit [Å]	1.600	1.600
Rmerge	0.090 *	0.080
Total number of observations	293876 *
Number of reflections	57378 *
<I/σ(I)>	9.7	2.5
Completeness [%]	99.9	99
Redundancy	5.2	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.25 *		used to seeding, Soisson, S.M., (1997) Science, 276, 421. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	18 (%)
2	1	reservoir	Tris-HCl	100 (mM)
3	1	reservoir	magnesium acetate	40 (mM)