2AA1
Crystal structure of the cathodic hemoglobin isolated from the Antarctic fish Trematomus Newnesi
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-04-07 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9393 |
Spacegroup name | P 1 |
Unit cell lengths | 58.097, 60.801, 61.232 |
Unit cell angles | 80.73, 81.36, 62.01 |
Refinement procedure
Resolution | 20.000 - 1.800 |
R-factor | 0.193 |
Rwork | 0.186 |
R-free | 0.22000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hbh |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | X-PLOR |
Data quality characteristics
Outer shell | |
Low resolution limit [Å] | 1.880 |
High resolution limit [Å] | 1.800 |
Completeness [%] | 77 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 298 | 9-12%(w/v) PEG 6000, phosphate buffer, pH 6.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K |