2A9I

Molecular Structure of the Interleukin-1 Receptor-Associated Kinase-4 Death Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-ID-B
Synchrotron site	APS
Beamline	14-ID-B
Temperature [K]	77
Detector technology	CCD
Collection date	2004-10-29
Detector	MARRESEARCH
Wavelength(s)	0.97888
Spacegroup name	C 1 2 1
Unit cell lengths	76.152, 38.043, 51.588
Unit cell angles	90.00, 121.07, 90.00

Refinement procedure

Resolution	18.000 - 1.700
R-factor	0.17982
Rwork	0.177
R-free	0.22255
Structure solution method	MAD
RMSD bond length	0.017
RMSD bond angle	1.719
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall
Low resolution limit [Å]	18.000
High resolution limit [Å]	1.700
Number of reflections	13974

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	281	100mM HEPES pH 7.5, 25% PEG 3350, 10 mM MnCl2 , VAPOR DIFFUSION, HANGING DROP, temperature 281K
1	VAPOR DIFFUSION, HANGING DROP	7.5	281	100mM HEPES pH 7.5, 25% PEG 3350, 10 mM MnCl2 , VAPOR DIFFUSION, HANGING DROP, temperature 281K