2A90

Crystal Structure of the tandem WWE domain of Drosophila Deltex

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	80
Detector technology	CCD
Collection date	2004-04-25
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9795, 0.9793, 0.9724
Spacegroup name	P 41
Unit cell lengths	57.760, 57.760, 80.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.800 - 2.150
Rwork	0.230
R-free	0.24700
Structure solution method	MAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.800	2.280
High resolution limit [Å]	2.150	2.150
Number of reflections	27871
Completeness [%]		99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	Sodium Acetate, Sodium Cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K