2A8X
Crystal Structure of Lipoamide Dehydrogenase from Mycobacterium tuberculosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X9A |
Synchrotron site | NSLS |
Beamline | X9A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-05-26 |
Detector | MARRESEARCH |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 83.582, 96.677, 122.889 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.940 - 2.400 |
R-factor | 0.1991 |
Rwork | 0.199 |
R-free | 0.24600 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.940 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Number of reflections | 72840 | |
<I/σ(I)> | 9.3 | 1.8 |
Completeness [%] | 96.6 | 92.3 |
Redundancy | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 279 | 50% MPD, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 279K |