2A8V
RHO TRANSCRIPTION TERMINATION FACTOR/RNA COMPLEX
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 110 |
Detector technology | IMAGE PLATE |
Collection date | 1998-04-15 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 132.783, 31.308, 105.751 |
Unit cell angles | 90.00, 122.98, 90.00 |
Refinement procedure
Resolution | 14.000 - 2.400 |
R-factor | 0.2455 |
Rwork | 0.245 |
R-free | 0.29700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1a8v |
RMSD bond length | 0.007 |
RMSD bond angle | 23.140 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 14.000 | 2.490 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.074 * | 0.283 * |
Number of reflections | 14393 | |
<I/σ(I)> | 16 | 3.6 |
Completeness [%] | 99.7 | 99.6 |
Redundancy | 3.7 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 8.5 * | 30 * | pH 4.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | reservoir | MPD | 10-20 (%) | |
3 | 1 | reservoir | ammonium acetate | 50-100 (mM) | |
4 | 1 | reservoir | Tris | 100 (mM) | pH8.5 |
5 | 1 | reservoir | 4 (mM) | ||
6 | 1 | reservoir | erbium acetate | 2 (mM) |