2A8T
2.1 Angstrom Crystal Structure of the Complex Between the Nuclear U8 snoRNA Decapping Nudix Hydrolase X29, Manganese and m7G-PPP-A
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 95 |
Detector technology | IMAGE PLATE |
Collection date | 2005-04-03 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 50.702, 81.710, 111.706 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.170 - 2.100 |
R-factor | 0.217 |
Rwork | 0.212 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u20 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.137 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | CNS |
Refinement software | REFMAC (5.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.170 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.037 | 0.380 |
Number of reflections | 27774 | |
<I/σ(I)> | 19.8 | 4.4 |
Completeness [%] | 99.9 | 100 |
Redundancy | 6.92 | 6.88 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.68 | 293 | 4-5 mg/ml X29, 0.025M HEPES pH 7.68, 3/75% PEG 6000, VAPOR DIFFUSION, SITTING DROP, temperature 293K |