2A8M
Crystal Structure of Human Taspase1 (T234S mutant)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-02-05 |
Detector | ADSC QUANTUM 4 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 60.441, 89.371, 104.276 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.590 - 2.600 |
R-factor | 0.207 |
Rwork | 0.207 |
R-free | 0.29000 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SnB (THEN SOLVE/RESOLVE) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.101 | 0.392 |
Number of reflections | 16867 | |
<I/σ(I)> | 15.0097 | 2.96 |
Completeness [%] | 93.6 | 73.1 |
Redundancy | 5.9 | 4.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6 | 294 | pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K |