2A8L

Crystal structure of Human Taspase1 (T234A mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2005-02-03
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	60.195, 85.750, 104.564
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.460 - 2.000
R-factor	0.205
Rwork	0.205
R-free	0.25300
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SnB (THEN SOLVE/RESOLVE)
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.075	0.415
Number of reflections	37258
<I/σ(I)>	21.6843	4.067
Completeness [%]	99.7
Redundancy	5.4	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	294	pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 294K