2A89
Monomeric Sarcosine Oxidase: Structure of a covalently flavinylated amine oxidizing enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-07-07 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.279, 69.059, 74.127 |
| Unit cell angles | 90.00, 94.13, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.850 |
| Rwork | 0.178 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1L9F |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.420 |
| Data scaling software | SCALEPACK |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.068 | 0.254 |
| Number of reflections | 55726 | |
| <I/σ(I)> | 12.4 | 2.4 |
| Completeness [%] | 89.2 | 49.4 |
| Redundancy | 3.85 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.7 | 296 | Na/K phosphate, pH 6.7, VAPOR DIFFUSION, SITTING DROP, temperature 296K |
