2A83
Crystal structure of hla-b*2705 complexed with the glucagon receptor (gr) peptide (residues 412-420)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-04-05 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.933 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.982, 82.083, 65.316 |
| Unit cell angles | 90.00, 108.73, 90.00 |
Refinement procedure
| Resolution | 28.990 - 1.400 |
| R-factor | 0.12734 |
| Rwork | 0.126 |
| R-free | 0.14955 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jge |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.480 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.039 | 0.112 |
| Number of reflections | 97993 | |
| Completeness [%] | 97.9 | 87.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 14% PEG4000,100mM Tris/HCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
