2A7W
Crystal Structure of Phosphoribosyl-ATP Pyrophosphatase from Chromobacterium violaceum (ATCC 12472). NESG TARGET CVR7
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-04-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97908,0.97933,0.96771 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 97.938, 67.544, 113.512 |
| Unit cell angles | 90.00, 92.45, 90.00 |
Refinement procedure
| Resolution | 29.310 - 2.800 |
| R-factor | 0.2582 |
| Rwork | 0.258 |
| R-free | 0.27990 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.000 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.079 | 0.382 |
| Number of reflections | 36861 | |
| <I/σ(I)> | 17.63 | 2.53 |
| Completeness [%] | 96.5 | 97.6 |
| Redundancy | 4.07 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |
