2A6Y

Crystal structure of Emp47p carbohydrate recognition domain (CRD), tetragonal crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2003-10-19
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	P 43 21 2
Unit cell lengths	70.310, 70.310, 100.140
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.420
R-factor	0.134
Rwork	0.134
R-free	0.19200
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	0.030
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.470
High resolution limit [Å]	1.420	1.420
Rmerge	0.058	0.374
Number of reflections	48001
<I/σ(I)>	14	3.6
Completeness [%]	99.6	99.9
Redundancy	13.6	13.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.1	277	Sodium dihydrogen phosphate, Dipotassium hydrogen phosphate, Lithium sulfate, CAPS, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 277K
1	VAPOR DIFFUSION, HANGING DROP	6.1	277	Sodium dihydrogen phosphate, Dipotassium hydrogen phosphate, Lithium sulfate, CAPS, pH 6.1, VAPOR DIFFUSION, HANGING DROP, temperature 277K