2A6Q

Crystal structure of YefM-YoeB complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2003-11-21
Detector	MARRESEARCH
Wavelength(s)	1.00000
Spacegroup name	P 64
Unit cell lengths	88.485, 88.485, 135.150
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.190 - 2.050
R-factor	0.225
Rwork	0.224
R-free	0.25400
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.280	2.090
High resolution limit [Å]	2.030	2.020
Number of reflections	38636
<I/σ(I)>	27.86
Completeness [%]	99.4	84.2
Redundancy	11.2	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	298	Tris-HCl, NaCl, DTT, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	7	298	Tris-HCl, NaCl, DTT, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	7	298	Tris-HCl, NaCl, DTT, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K
1	VAPOR DIFFUSION, SITTING DROP	7	298	Tris-HCl, NaCl, DTT, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K