2A6J
Crystal structure analysis of the anti-arsonate germline antibody 36-65
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-04-08 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.137, 73.955, 85.922 |
| Unit cell angles | 90.00, 113.92, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.700 |
| R-factor | 0.228 |
| Rwork | 0.225 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.791 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.095 | |
| Number of reflections | 23857 | |
| <I/σ(I)> | 6.91 | 1.86 |
| Completeness [%] | 85.3 | 86.7 |
| Redundancy | 2.54 | 1.52 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 298 | 18% PEG 8000, cacodylate, 0.05% azide , pH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 298.0K |
